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Additionally, the complex in vivo environment in humans is difficult to replicate in laboratory settings

coli BL21 (DE3), which add the His-tag (His x6 ) at the carboxy-terminal domain of the GpoA protein to facilitate its purification

LKB1, a tumor suppressor, is a major mammalian AMPK kinase in the liver that activates AMPK by phosphorylating the Thr172 residues of AMPK subunits

Sermorelin (GHRH 1-29 NH2) is a synthetic 29-amino acid peptide corresponding to the N-terminal fragment of endogenous GHRH