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Step 2: The enzyme glutathione synthetase (GS) adds glycine to gamma-glutamylcysteine, completing the synthesis of glutathione

Unresolved hypotheses: mechanistic proposals that remain plausible but have not been directly demonstrated in the relevant AOD9604 metabolic model

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The reasons for these changes, apart from excess ROS leading to increased GSH depletion in cardiomyocytes, are disturbances in the anabolic process of GSH, causing a further decrease in intracellular GSH content

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