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The crucial role of methodology development in directed evolution of selective enzymes

For example, branched-chain amino acids (BCAAs) promote inflammation-related metabolic programs through transporters and mechanistic target of mTORC1 (mTORC1) signaling, whereas under conditions of amino acid deprivation or energetic stress, starvation sensing mediated by GCN2 can restrict inflammasome activation and IL-1 production, causing supply and restriction to generate opposite immune outputs at different thresholds (54)

A dual-emissive-materials design concept enables tumour hypoxia imaging

Diabetes Care 25 , 11351141 (2002)

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Carbohydr Polym 196:233245