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Molecular features of the ligand-free GLP-1R, GCGR and GIPR in complex with G(s) proteins

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To transform the library plasmid into the desired selection strain, selection strains (S2060 (pBAD-protease) derivatives) were grown overnight in 5 ml of DRM supplemented with 50 g ml 1 spectinomycin and 12 g ml 1 tetracycline

Vitamin C regenerates oxidized glutathione back to its active form, supports collagen synthesis, suppresses excess melanin formation, and enhances overall antioxidant capacity