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Heres my honest take
Injections of 1 ml/kg volume were made once a day between 8 and 11 a.m
W., & Ali, A
Methods Animals Male C57BL/6J mice at 57 weeks of age were used for the purpose of pancreatic islets isolation
Those two changes do two things: they prevent binding to IGFBP (the carrier proteins that normally inactivate free IGF-1), and they extend half-life from minutes to roughly 24 hours
Older secretagogues like GHRP-6 and GHRP-2 also activated this pathway, but they came with significant side effects: hunger, cortisol spike, prolactin elevation
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